Thermal Sheath & Temperature-controlled incubations

This page is under construction, please reach out to our team for help with the thermal sheath and temperature modules.

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Congrats on choosing the Omni-Stainer™ thermal sheath S12 / C12! The Omni-Stainer™ is made of heat-conductive aluminum and can be used in conjunction with a temperature control module, allowing the temperature to be programmatically controlled between -20° to 99.9°C. This enables a variety of protocols, such as FFPE Antigen Retrieval, smRNA-FISH, or CycIF to be performed directly inside the Omni-Stainer™ device. To run thermal incubations there are 3 main things you need

1. A thermal module. We recommend QInstruments ColdPlate

2. A temperature-controlled program

3. A thermal sheath and Opentrons robot

Omni-Stainer going onto thermal module and covered with thermal sheath

QInstruments ColdPlate Product Page

Temperature-controlled protocols

• Assemble Omni-Stainer as in the Assembly and Sample Prep protocols, make sure there is sufficient water in the base and the humidity barrier paper.

  • S12 Assembly and Sample Prep

  • C12 Assembly and Sample Prep

• Place thermal sheet on top of the Omni-Stainer

• Set up the temperature module on the deck of the QInstruments ColdPlate manual.

• When running the protocols, make sure to select “omni_stainer_###_with_thermosheath”, where ### is the instrument model (i.e. S12_slides or C12_cslps)

• During the calibration step, calibrate the pipette tip to the calibration point on top of the thermal sheath

When creating your own protocol, ensure that you utilize theopenShutter(...), and closeShutter(...) functions from the protocol header. Neglecting to do so may result in damage to your pipette, for which we cannot be held responsible.

Temperature Modules

At Parhelia , we value efficient, precise equipment. That's why for the Opentrons OT-2 to effectively utilize the Omni-Stainer S12 or C12 with a thermal sheath, we recommend the QInstruments thermal module. The current Opentrons thermal module is slightly too tall for the Omni-Stainer with thermal sheath, making QInstruments the ideal alternative. Their ColdPlate ensures unrivaled heating and cooling precision for demanding robotic applications. QInstruments ColdPlate Product Page

QInstruments ColdPlate

Compact, lightweight, effective, and silent, it provides heating and cooling combined in a single unit. Fully adjustable from -20° to 99.9°C, its optimized thermo adapters and premium aluminium housing ensure excellent temperature control. Opentrons support remains available for assistance, despite this being a third-party module. With an easy setup and an extended warranty, the QInstruments module is a valuable addition to any laboratory setup.

If you have any questions, comments, or concerns please email [e-mail]