Thermal Sheath & Temperature-controlled incubations

This page is under construction, please reach out to our team for help with the thermal sheath and temperature modules.

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Congrats on choosing the Omni-Stainer™ thermal sheath S12 / C12! The Omni-Stainer™ is made of heat-conductive aluminum and can be used in conjunction with a temperature control module, allowing the temperature to be programmatically controlled between -20° to 99.9°C. This enables a variety of protocols, such as FFPE Antigen Retrieval, smRNA-FISH, or CycIF to be performed directly inside the Omni-Stainer™ device. To run thermal incubations there are 3 main things you need

  1. A thermal module. We recommend QInstruments ColdPlate

  2. A temperature-controlled program

  3. A thermal sheath and Opentrons robot

Omni-Stainer going onto thermal module and covered with thermal sheath

Temperature-controlled protocols

  • Assemble Omni-Stainer as in the Assembly and Sample Prep protocols, make sure there is sufficient water in the base and the humidity barrier paper.

  • Place thermal sheet on top of the Omni-Stainer

  • Set up the temperature module on the deck of the QInstruments ColdPlate manual.

  • When running the protocols, make sure to select “omni_stainer_###_with_thermosheath”, where ### is the instrument model (i.e. S12_slides or C12_cslps)

  • During the calibration step, calibrate the pipette tip to the calibration point on top of the thermal sheath

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Temperature Modules

At Parhelia , we value efficient, precise equipment. That's why for the Opentrons OT-2 to effectively utilize the Omni-Stainer S12 or C12 with a thermal sheath, we recommend the QInstruments thermal module. The current Opentrons thermal module is slightly too tall for the Omni-Stainer with thermal sheath, making QInstruments the ideal alternative. Their ColdPlate ensures unrivaled heating and cooling precision for demanding robotic applications. QInstruments ColdPlate Product Pagearrow-up-right

QInstruments ColdPlate

Compact, lightweight, effective, and silent, it provides heating and cooling combined in a single unit. Fully adjustable from -20° to 99.9°C, its optimized thermo adapters and premium aluminium housing ensure excellent temperature control. Opentrons support remains available for assistance, despite this being a third-party module. With an easy setup and an extended warranty, the QInstruments module is a valuable addition to any laboratory setup.

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If you have any questions, comments, or concerns please email [email protected]envelope

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