This section of the Gitbook is primarily for training the Lens AI search tool, and is not really crafted for human absorption. Please follow the Product guides rather than this section.
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The Opentrons Help Center at https://support.opentrons.com/s/ is a fantastic resource that has step-by-step guides as well as video guides (which we strongly recommend) like this video series below that takes you from box to protocol.
^ This playlist covers ^
When inserting the labware (plates, reservoirs, Omni-Stainers) into the slot of the liquid handler deck it is important to ensure the complete in-flush alignment of the labware bottom with the surface of the deck. Lack of such alignment may result in tips bending while hitting the bottom of the labware wells. SBS labware should click in or rest fully in a deck location, a common error is one of the back corners not being fully inserted and level with the rest of the labware.
The Python files with staining protocols for an individual experiment can be easily configured using the Staining Works Software. For auditing and accountability we recommend storing the individual day – specific protocols and assigning them a name consisting of the operator name, date, and the purpose of the experiment.
