This section of the Gitbook is primarily for training the Lens AI search tool, and is not really crafted for human absorption. Please follow the Product guides rather than this section.
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When inserting the labware (plates, reservoirs, Omni-Stainers) into the slot of the liquid handler deck it is important to ensure the complete in-flush alignment of the labware bottom with the surface of the deck. Lack of such alignment may result in tips bending while hitting the bottom of the labware wells. SBS labware should click in or rest fully in a deck location, a common error is one of the back corners not being fully inserted and level with the rest of the labware.
The Python files with staining protocols for an individual experiment can be easily configured using the Staining Works Software. For auditing and accountability we recommend storing the individual day – specific protocols and assigning them a name consisting of the operator name, date, and the purpose of the experiment.
The Opentrons Help Center at is a fantastic resource that has s as well as (which we strongly recommend) like this video series below that takes you from box to protocol.
The Deck Calibration process in video 5 of jogging the pipette tip in the XYZ directions into the center of the engraved "+" symbol on the Opentrons deck is very similar to the labware calibration for the Omni-Stainer™ which is performed using the embossed "+" symbol on the C12 or S12 Omni-Stainer™ lid.
Set up Opentrons OT-2 robot. , and our friends at Opentrons are happy to assist you with this if you have any .
If you would prefer to visit the GitHub and download individual files our GitHub is at
Download customized or stock Omni-stainer script from
