GetstartedImportaprotocol
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OpenTrons. (2023). Get started: Import a protocol. OpenTrons Support. Retrieved from
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After you’ve attached pipettes and finished deck, tip length, and pipette offset calibrations, you can import a protocol.
In the Opentrons App, select the Protocols tab. If you have not imported any protocols, you will be able to select Choose File or drag and drop to import your Python (.py) or Protocol Designer (.json) file.
If you have already imported a protocol, this is also where you will see it listed.
When a protocol is imported into the app, it undergoes protocol analysis local to the Opentrons App (this is before it is simulated on the OT-2 itself). This process extracts information from the protocol and loads it into the app to display the metadata and graphics.
To proceed, select the protocol you want to run.
Note: You can select Import to add more protocols. You can also change the way the list is organized by selecting a different option from the Sort by dropdown menu in the upper right.
After selecting your protocol, you'll be taken to the protocol overview page. Select Run protocol to continue setting up your run.
You will be prompted to choose an available robot before you can select Proceed to setup.
If this is your first time connecting to the robot, please see:
If you've successfully connected to the robot before but are suddenly unable to, please see:
Details about your protocol are broken down into 3 tabs on the run setup page:
Setup
Module Controls
Run Preview
The Setup tab displays the statuses of Robot Calibration, Module Setup (if your protocol uses Opentrons modules), Labware Setup, and Liquid Setup. Click on a step to expand it.
Note: If your protocol does not use any modules, the Module Setup step will not be shown.
Robot Calibration allows you to review current calibration data and will flag any required calibrations that are missing or need to be redone.
Module Setup displays information about the modules used in the protocol.
Initial Liquid Setup displays the volume and starting location of each liquid used in the protocol. Click on a liquid to further expand it.
You can click on an expanded liquid's details to see specific information about the labware containing the liquid, as well as its starting deck state.
The Module Controls tab allows you to interact with connected Opentrons modules that will be used in your protocol.
The protocol in the example shown throughout this article uses a Magnetic Module. You can set the engage height of the magnets by selecting the three-dot menu (⋮) > Set engage height.
The Run Preview tab displays a preview of your protocol's steps.
When you're ready to run your protocol, select Start run at the top.
Copyright © 2023 Opentrons
If you haven't yet decided on a protocol method, see .
If you are using the Python API and see an error such as “Module not found” for Python packages or “File not found” for csv files loaded onto your OT-2, please review the troubleshooting steps found in .
Labware Setup displays information about labware used in the protocol. This is also where you can run Labware Position Check. See for additional details.
