☑️C12 Assembly and Sample Prep
How to put together your Omni-Stainer™ C12 system and prepare samples for staining
Step 1 - Create Humid Environment: add 30 mL deionized water to the Omni-Stainer™ C12 base
Step 2 - Construct Flow Cell and Place in Base
You can create a flow cell in two methods:
[Shown in video below] Add ~100 ul of PBS on the Support Pad, and then place the coverslip (sample side down) onto the support pad. Adjust the coverslip ensuring it aligns with the lower boundary of the Support Pad. If there are air bubbles, sliding the coverslip such that bubble is released and then sliding it back after adding a bit more of the liquid allows to "expel" the bubbles
Align the coverslip and Support Pad "underwater" within a PBS pool, this technique that demands less skill to avoid air bubbles - however, the delicacy and clearness of coverslips occasionally make this less suitable for the C-style flow cell than for the S-style.
Step 3 - Confirm Capillary Connection
It is important to verify that the coverslip aligns perfectly with the lower boundary of the Support Pad and makes contact with the wicking pillar. Confirm the existence of a capillary connection between the staining chamber and the wicking pillar, which essentially entails a liquid droplet linking the two components. If that’s not the case, dispense 100ul of PBS onto the contact point between the wicking pillar and the support tile (see diagram below).
Step 4 - Adhere moisture barrier paper to the lid's bottom, wet it with 10 mL of deionized water
Step 5 - Put the lid on the base, matching slanted corners
Full Assembly
Using a Thermal Sheath?
Thermal Sheath & Temperature-controlled incubationsDone! You're now ready to choose Manual or Automated mode and launch the demo!
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