# Additional materials

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The materials below are only required for automated operation. **For manual operation, you only need a micropipette + tips, and your samples.**
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<figure><img src="https://3271682270-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FWKyUXOqIKtqE7cAUE2yQ%2Fuploads%2Fs8Ev4vg2FwvdAaURaPku%2FOmni%20Stainer%20in%20OT-2%20with%20custom%20labwares.jpeg?alt=media&#x26;token=7c11d56c-99da-4382-abec-7b3bdcc9db7d" alt=""><figcaption></figcaption></figure>

Additional Parhelia labware shown above with the [Parhelia Omni-Stainer™ S12 Module Automation Bundle](https://parheliabio.com/product/omni-stainer-s12-automation-bundle/?utm_source=gitbook\&utm_medium=gitbook), in the [OT-2 robot](https://parheliabio.com/product-category/opentrons-robot-purchase/?utm_source=gitbook\&utm_medium=gitbook) with [Gen2 P300 Single-Channel](https://parheliabio.com/product/opentrons-single-channel-electronic-pipette-p300/?utm_source=gitbook\&utm_medium=gitbook) and [Opentrons 300 µl Pipette Tips](https://parheliabio.com/product/opentrons-300-ul-pipette-tip-rack-and-refill/?utm_source=gitbook\&utm_medium=gitbook) performing 10X Visium H\&E staining @ The Broad Institute.

## [Parhelia Biosciences Reagent Reservoir Starter Kit](https://parheliabio.com/product/parhelia-biosciences-reagent-reservoir-starter-kit/?utm_source=gitbook\&utm_medium=gitbook)

The Starter Kit contains all the additional consumables needed to stain your samples, besides reagents, slides/coverslips, and [pipette tips](https://parheliabio.com/product/opentrons-300-ul-pipette-tip-rack-and-refill/?utm_source=gitbook\&utm_medium=gitbook), in a convenient quantity.

<figure><img src="https://3271682270-files.gitbook.io/~/files/v0/b/gitbook-x-prod.appspot.com/o/spaces%2FWKyUXOqIKtqE7cAUE2yQ%2Fuploads%2FTnkdlFApVfV25GDRFSam%2FReagent%20Reservoir%20Starter%20Kit%20Image%20enhanced.png?alt=media&#x26;token=bb1a4d0a-b91d-4479-98cf-ad730ebff63b" alt=""><figcaption><p><a href="https://parheliabio.com/product/parhelia-biosciences-reagent-reservoir-starter-kit/?utm_source=gitbook&#x26;utm_medium=gitbook">Parhelia Biosciences Reagent Reservoir Starter Kit</a> contains 3 X Deep-Well 12-Trough Reservoirs, 10 X Skirted 96-Well PCR Plates, and 50 X Pierceable Sealing Films.</p></figcaption></figure>

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It's crucial to avoid using unvalidated labware as it can potentially result in notable automation errors.
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Obtain an [Parhelia Biosciences Reagent Reservoir Starter Kit](https://parheliabio.com/product/parhelia-biosciences-reagent-reservoir-starter-kit/?utm_source=gitbook\&utm_medium=gitbook) to get up and staining quickly!
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<details>

<summary>Pierceable aluminum foil seals</summary>

Peelable plate seals are highly beneficial for preparing and storing reservoirs. Specifically, aluminum foil can be pierced by pipette tips, eliminating the need to peel off the seal, which could otherwise lead to unwanted well contamination or spillage. Additionally, foil seals protect light-sensitive reagents. \
\
Pro-tip: If you are going to store sealed plates use a [roller tool ](https://www.thomassci.com/Molecular-Diagnostics/Automation/Plates/Sealing/_/Sealing-Film-Accessories)to really make sure the edges are sealed tightly or else you may get evaporation!

</details>

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If you have any questions, comments, or concerns with your Parhelia products please email [info@parheliabio.com](https://app.gitbook.com/u/hZa0JIMxOpQOLaWee4XzjrlnFyX2)
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