Omni-stainer user guide
  • ▶️Overview
  • Product Guides
    • 🥳Quick Start: Omni-Stainer™ Bundles
    • 📦Omni-Stainer™ Box Contents
    • 🛍️Additional materials
    • ☑️S12 Assembly and Sample Prep
    • ☑️C12 Assembly and Sample Prep
    • 🥇Sample Mounting Instructions
    • 🧑‍🔬Manual Operation and "Blue Dye Demo" Manual Protocol
    • 🤖Automation (Opentrons OT-2) Setup
      • 📋Omni-Stainer Automation Overview
      • 📥Importing labware definitions
        • 🆕Opentrons App v7 Updated Labware Definitions
      • ⛱️StainWorks
      • 🗺️Labware Layouts
      • Thermal Sheath & Temperature-controlled incubations
    • 💙"Blue Dye Demo" Automated Protocol
    • 💾Software Requirements
  • Protocol Knowledge Base
    • PhenoCycler® (CODEX)
    • Gram Stain
    • IF
    • IHC
    • H&E
    • RNAScope
    • Opal
    • IMC
  • Background Knowledge, LLM semantic training data
    • 🗄️Background info and Lens training docs
      • Importing labware definitions
      • StainWorks software: a centralized app for accessing and configuring protocols
      • Reagent Layout files
      • Running the "Blue Dye Demo" protocol
      • Copy of CODEX (PhenoCyler™)
        • CODEX Reagent Setup
      • Temperature-controlled incubations
      • Page 2
      • ParheliaBio.com content
        • Advanced Tissue Staining Solutions | ParheliaBio
        • Omni-Stainer™ S12 - Automation Bundle
        • Omni-Stainer™ S12 - Manual Operation Bundle
        • Shop
        • Omni-Stainer™ S12 Thermal Sheath
        • Omni-Stainer™ C12 - Automation Bundle
        • Omni-Stainer™ C12 Thermal Sheath
        • Consumables Archives
        • Conditioning Reagent 5ml Bottle
        • Omni-Stainer™ C12 - Manual Operation Bundle
        • S12 Coverpads - 48 Pack
        • C12 Filter Paper
        • Opentrons Robots Archives
        • S12 Filter Paper
        • C12 Support Pad - 48 Pack
        • Opentrons OT-2 Liquid Handling Robot
        • Opentrons Single-Channel Electronic Pipette p20 (GEN2)
        • Opentrons 300 µl Pipette Tip Tip Racks - 9600 tips
        • Greater Tissue Staining Workflow Efficiency. Slide Staining Kits
      • Opentrons setup guides
        • Opentronssetupguides
        • GetstartedImportaprotocol
      • More Parheliabio.com content
        • Howitworks
        • Parhelia Biosciences FAQs
          • Page 1
            • Frequently asked questions parheliabio website
        • ParheliaFrequentlyaskedquestions
        • Parhelia Omni-Stainer FAQ
      • Meet your Parhelia Omnistainer
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On this page
  • How it works
  • Peace of mind of having reproducible results – with simple or complex protocols
  • Omni-Stainer™ C12
  • How it works
  • Omni-Stainer™ S12
  • How it works
  • Placing slides into Omni-Stainer™
  • Manual staining
  • Automated Staining Demo
  • Liquid handling
  • Heating and cooling on the robot: FFPE-IHC

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  1. Background Knowledge, LLM semantic training data
  2. Background info and Lens training docs
  3. More Parheliabio.com content

Howitworks

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Last updated 1 year ago

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PRECISION TISSUE STAINING EVERY TIME

AT THE PUSH OF A BUTTON

How it works

Greater workflow efficiency - saves time and reduces the need for dedicated personnel and training - automated liquid handlers are faster than manual pipetting.

MAKING TISSUE AUTO-STAINING ACCESSIBLE TO ALL

Peace of mind of having reproducible results – with simple or complex protocols

Omni-Stainer™ C12

for coverslips

  • Compact: footprint of a 96-well plate

  • Stains up to 12 coverslip-mounted samples at a time

  • Saves reagents: 50% less reagents compared to open-top staining

  • Never lose or break a sample

  • Built-in wet chamber

  • Use it by hand or insert into any SBS-compatible liquid handler to automate your staining

How it works

for coverslips

  1. Coverslip with sample rests on 50um thin spacers, forming a capillary chamber. Staining reagents are supplied by a liquid handler (or by hand!)

  2. Sample is incubated in a thin capillary layer

  3. When the sample is washed, the old one is instantly displaced by the laminar flow

Omni-Stainer™ S12

for slides

  • Compact: footprint of a 96-well plate

  • Stains up to 12 coverslip-mounted samples at a time

  • Saves reagents: 50% less reagents compared to open-top staining

  • Never lose or break a sample

  • Built-in wet chamber

  • Use it by hand or insert into any SBS-compatible liquid handler to automate your staining

How it works

for slides

  • The same capillary displacement principle, but the sample is mounted on a slide and covered with a coverpad that incorporates capillary spacers

EFFICIENT - ACCURATE - AFFORDABLE

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Placing slides into Omni-Stainer™

Slide assembly This video shows how to assemble the slide/coverpad sandwich and place it into the slide Omni-StainerTM. First the tacky spacers are exposed by removing the protective tape from the cover pad. To avoid airbubbles between the slide and the coverpad the slide is first placed in the small tray with compatible buffer (water or PBS) and in such submerged stage is covered with a coverpad. The spacers are tacky enough to hold the coverpad in place (after slightly pressed against the slide) but yet when wet allow repositioning the coverpad over the slide. After the sandwich is assembled the combo is placed on the shelf and then into the Omni-StainerTM

Play Video

Coverslip Assembly This video shows how to assemble the coverslip/support pad sandwich and place it into the coverslip Omni-StainerTM system. To avoid airbubbles between the slide and the support pad, a drop of compatible buffer (water or PBS) is first placed onto the support pad. The coverslip with a sample is then placed face down on the pad to avoid bubble formation and ensure that the liquid fully fills the flow cell formed by sandwiching the support pad and the coverslip. After the sandwich is assembled the combo is placed into the coverslip version of the Omni-StainerTM system.

Play Video

Manual staining

Using Omni-Stainer™ platform by hand

Play Video

Omni-Stainer™ S12 platform Demo Video

Play Video

Omni-Stainer™ C12 platform Demo Video

The Omni-StainerTM platform can be used as a device for manual staining. In such case the solutions are exchanged by manual pipetting. After the pipetting the Omni-StainerTM system should be closed with a lid. In such configuration it can be placed into the 4C refrigerator or into the heated incubator. Before the start of any staining experiment 20-30ml of water should be placed into the Omni-StainerTM system to create the humid chamber environment which helps to reduce the evaporation of staining solutions from the slide/coverpad or coverslip/support pad sandwich.”

Automated Staining Demo

using Opentrons OT-2

Playback speed increased^

Omni-Stainer™ S12 platform automation demo

Omni-Stainer™ C12 platform Demo Video

For automated liquid exchange the Omni-Stainer™ system has to be place into programmable liquid handler (e.g. Opentrons). The design of the lid including the prewetted perforated filter paper on the lid ensures minimizing the evaporation of staining reagents from the slide/coverpad or coverslip/support pad sandwich.

Liquid handling

OT-2

Staining automation can be attained by inserting the Omni-Stainer™ into an SBS-compatible liquid handler, and how we are partnering with Opentrons and adopting their versatile ultra-low-cost liquid robot OT-2 to deliver complete staining automation solutions.

Omni-Stainer™ is compatible with most liquid handling robots but if you don't have one, we recommend Opentrons OT-2

Protocols Supported on OT-2:

  • IF

  • IHC

  • CODEX single and multi-strain

  • Cas-FISH

  • FFPE Antigen Retrieval (HIER)

  • Opal

Coming soon:

  • smRNA-FISH

  • Rolling Circle Amplification

  • Cyclic IF

  • IMC & MIBI

  • and many more...

Heating and cooling on the robot: FFPE-IHC

Omni-Stainer™ system rests on top of the OT-2 thermal module after: Human tonsil FFPE antigen retrieval at 95°C Anti-CD8 Primary antibody staining at 4°C Secondary antibody staining, DAB and hematoxylin at room temperature

Are you ready for the Omni-Stainer™ platform to transform your life?

As a part of our ‘’, we provide pre-written protocols for various assays, including H&E, IHC, IF, CycIF, CODEX, FFPE antigen retrieval, CAS-FISH, IMC, RNAscope.

© 2023 Parhelia Biosciences | | | |

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Omni-Stainer™ S12 platform
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Virtual Demo
OT 2
Heating and cooling on the robot: FFPE-IHC