Roadmap for Getting Set up with the Omni-Stainer in an Opentrons OT-2
Example of staining protocol loaded into Opentrons
We suggest starting with the "Blue Dye Demo" Automated Protocol before exploring other protocols. This demo's steps are crafted to generally adapt to various Parhelia protocols. Find the protocol here:
Our engaging virtual demonstration serves as an excellent introductory guide for understanding the steps in operating the Parhelia Bio Omni-Stainer C12 and S12, encompassing both manual and automated usage, along with thermal sheath and thermal modules. View the virtual demo here!
Step 1 - Place the Omni-Stainer, reagent reservoirs, pipette tip rack, and antibody plate into the dedicated slots on the deck of the liquid handler.
When inserting the labware (plates, reservoirs, Omni-Stainers) into the slot of the liquid handler deck it is important to ensure the complete in-flush alignment of the labware bottom with the surface of the deck. Lack of such alignment may result in tips bending while hitting the bottom of the labware wells. SBS labware should click in or rest fully in a deck location, a common error is one of the back corners not being fully inserted and level with the rest of the labware.
Step 2 - Create a running protocol using the Staining Works Software
The Python files with staining protocols for an individual experiment can be easily configured using the Staining Works Software. For auditing and accountability we recommend storing the individual day–specific protocols and assigning them a name consisting of the operator name, date, and the purpose of the experiment.
For more information go to our StainWorks documentation page:
You may see a protocol analysis failure in the Opentrons App if the necessary labware definitions have not been uploaded. To learn more see this documentation page:
