📋Omni-Stainer Automation Overview
Roadmap for Getting Set up with the Omni-Stainer in an Opentrons OT-2
Last updated
Roadmap for Getting Set up with the Omni-Stainer in an Opentrons OT-2
Last updated
Download customized or stock Omni-stainer code from Stainworks
Load protocols and labware definitions into Opentrons software
Follow protocol-specific instructions, for preparing and loading your labware and doing protocol setup. We strongly recommend starting with the Blue Dye Demo protocol to get comfortable using the device before conducting actual experiments.
Our engaging virtual demonstration serves as an excellent introductory guide for understanding the steps in operating the Parhelia Bio Omni-Stainer C12 and S12, encompassing both manual and automated usage, along with thermal sheath and thermal modules. View the virtual demo here!
When inserting the labware (plates, reservoirs, Omni-Stainers) into the slot of the liquid handler deck it is important to ensure the complete in-flush alignment of the labware bottom with the surface of the deck. Lack of such alignment may result in tips bending while hitting the bottom of the labware wells. SBS labware should click in or rest fully in a deck location, a common error is one of the back corners not being fully inserted and level with the rest of the labware.
The Python files with staining protocols for an individual experiment can be easily configured using the Staining Works Software. For auditing and accountability we recommend storing the individual day–specific protocols and assigning them a name consisting of the operator name, date, and the purpose of the experiment.
For more information go to our StainWorks documentation page:
You may see a protocol analysis failure in the Opentrons App if the necessary labware definitions have not been uploaded. To learn more see this documentation page: