Precision tissue staining every time at the push of a button
Greater workflow efficiency - saves time and reduces the need for dedicated personnel and training - automated liquid handlers are faster than manual pipetting.
making tissue auto-staining accessible to all
for coverslips
Compact: footprint of a 96-well plate
Stains up to 12 coverslip-mounted samples at a time
Saves reagents: 50% less reagents compared to open-top staining
Never lose or break a sample
Built-in wet chamber
Use it by hand or insert into any SBS-compatible liquid handler to automate your staining
for coverslips
Coverslip with sample rests on 50um thin spacers, forming a capillary chamber. Staining reagents are supplied by a liquid handler (or by hand!)
Sample is incubated in a thin capillary layer
When the sample is washed, the old one is instantly displaced by the laminar flow
for slides
Compact: footprint of a 96-well plate
Stains up to 12 coverslip-mounted samples at a time
Saves reagents: 50% less reagents compared to open-top staining
Never lose or break a sample
Built-in wet chamber
Use it by hand or insert into any SBS-compatible liquid handler to automate your staining
The same capillary displacement principle, but the sample is mounted on a slide and covered with a coverpad that incorporates capillary spacers
EFFICIENT - ACCURATE - AFFORDABLE
Complete your details and view our virtual demonstration of the Omni-Stainer™
Slide assembly This video shows how to assemble the slide/coverpad sandwich and place it into the slide Omni-StainerTM. First the tacky spacers are exposed by removing the protective tape from the cover pad. To avoid airbubbles between the slide and the coverpad the slide is first placed in the small tray with compatible buffer (water or PBS) and in such submerged stage is covered with a coverpad. The spacers are tacky enough to hold the coverpad in place (after slightly pressed against the slide) but yet when wet allow repositioning the coverpad over the slide. After the sandwich is assembled the combo is placed on the shelf and then into the Omni-StainerTM
Coverslip Assembly This video shows how to assemble the coverslip/support pad sandwich and place it into the coverslip Omni-StainerTM system. To avoid airbubbles between the slide and the support pad, a drop of compatible buffer (water or PBS) is first placed onto the support pad. The coverslip with a sample is then placed face down on the pad to avoid bubble formation and ensure that the liquid fully fills the flow cell formed by sandwiching the support pad and the coverslip. After the sandwich is assembled the combo is placed into the coverslip version of the Omni-StainerTM system.
Using Omni-Stainer™ platform by hand
Omni-Stainer™ S12 platform Demo Video
Omni-Stainer™ C12 platform Demo Video
The Omni-StainerTM platform can be used as a device for manual staining. In such case the solutions are exchanged by manual pipetting. After the pipetting the Omni-StainerTM system should be closed with a lid. In such configuration it can be placed into the 4C refrigerator or into the heated incubator. Before the start of any staining experiment 20-30ml of water should be placed into the Omni-StainerTM system to create the humid chamber environment which helps to reduce the evaporation of staining solutions from the slide/coverpad or coverslip/support pad sandwich.”
using Opentrons OT-2
Omni-Stainer™ S12 platform automation demo
Omni-Stainer™ C12 platform Demo Video
For automated liquid exchange the Omni-Stainer™ system has to be place into programmable liquid handler (e.g. Opentrons). The design of the lid including the prewetted perforated filter paper on the lid ensures minimizing the evaporation of staining reagents from the slide/coverpad or coverslip/support pad sandwich.
Staining automation can be attained by inserting the Omni-Stainer™ into an SBS-compatible liquid handler, and how we are partnering with Opentrons and adopting their versatile ultra-low-cost liquid robot OT-2 to deliver complete staining automation solutions.
As a part of our ‘automation package’, we provide pre-written protocols for various assays, including H&E, IHC, IF, CycIF, CODEX, FFPE antigen retrieval, CAS-FISH, IMC, RNAscope. Custom protocol development services are available.
Omni-Stainer™ is compatible with most liquid handling robots but if you don't have one, we recommend Opentrons OT-2
Protocols Supported on OT-2:
IF
IHC
CODEX single and multi-strain
Cas-FISH
FFPE Antigen Retrieval (HIER)
Opal
Coming soon:
smRNA-FISH
Rolling Circle Amplification
Cyclic IF
IMC & MIBI
and many more...
Omni-Stainer™ system rests on top of the OT-2 thermal module after: Human tonsil FFPE antigen retrieval at 95°C Anti-CD8 Primary antibody staining at 4°C Secondary antibody staining, DAB and hematoxylin at room temperature
What people are saying...
With the Parhelia Omni-Stainer I’ve been able to increase the number of coverslips or slides I can do CODEX staining at the same time and, on top of that, I’ve been able to save reagents and antibody while preserving staining quality. Using their product was a great investment for our project
Washington University in St. Louis School of Medicine
We were very excited to be an early-access adopter of Parhelia’s automated coverslip staining solution. Their product significantly improved the reproducibility, labor, and throughput of our smFISH and CasFISH workflows. The Parhelia team provided easy to implement software scripts to run the protocols on the OT-2, and were very responsive in answering any of our questions as we were first setting up the process. Finally, the low upfront capital acquisition cost versus other cell staining automation solutions makes it very easy to justify the ROI.
Dahlia Biosciences - Co-Founder and CEO
I am not so sure about this Parhelia thing… I’d rather buy a machine from a big vendor that takes up the whole room, guzzles gallons of proprietory antibodies per run and costs 50X more.
Ever
