Automation (Opentrons OT-2) Setup
This page goes through setting up a new Opentrons OT-2, most of the content is in links to Opentrons website and Opentrons videos.
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This page goes through setting up a new Opentrons OT-2, most of the content is in links to Opentrons website and Opentrons videos.
Last updated
Was this helpful?
^ This playlist covers ^
Load protocols and labware definitions into Opentrons software
Follow protocol-specific instructions, for preparing and loading your labware and doing protocol setup. We strongly recommend starting with the Blue Dye Demo protocol to get comfortable using the device before conducting actual experiments.
The Opentrons Help Center at is a fantastic resource that has s as well as (which we strongly recommend) like this video series below that takes you from box to protocol.
The Deck Calibration process in video 5 of jogging the pipette tip in the XYZ directions into the center of the engraved "+" symbol on the Opentrons deck is very similar to the labware calibration for the Omni-Stainer™ which is performed using the embossed "+" symbol on the C12 or S12 Omni-Stainer™ lid.
Our engaging virtual demonstration serves as an excellent introductory guide for understanding the steps in operating the Parhelia Bio Omni-Stainer C12 and S12, encompassing both manual and automated usage, along with thermal sheath and thermal modules.
Set up Opentrons OT-2 robot. , and our friends at Opentrons are happy to assist you with this if you have any .
If you would prefer to visit the GitHub and download individual files our GitHub is at
Download customized or stock Omni-stainer script from