Omni-stainer user guide
  • ▶️Overview
  • Product Guides
    • 🥳Quick Start: Omni-Stainer™ Bundles
    • 📦Omni-Stainer™ Box Contents
    • 🛍️Additional materials
    • ☑️S12 Assembly and Sample Prep
    • ☑️C12 Assembly and Sample Prep
    • 🥇Sample Mounting Instructions
    • 🧑‍🔬Manual Operation and "Blue Dye Demo" Manual Protocol
    • 🤖Automation (Opentrons OT-2) Setup
      • 📋Omni-Stainer Automation Overview
      • 📥Importing labware definitions
        • 🆕Opentrons App v7 Updated Labware Definitions
      • ⛱️StainWorks
      • 🗺️Labware Layouts
      • Thermal Sheath & Temperature-controlled incubations
    • 💙"Blue Dye Demo" Automated Protocol
    • 💾Software Requirements
  • Protocol Knowledge Base
    • PhenoCycler® (CODEX)
    • Gram Stain
    • IF
    • IHC
    • H&E
    • RNAScope
    • Opal
    • IMC
  • Background Knowledge, LLM semantic training data
    • 🗄️Background info and Lens training docs
      • Importing labware definitions
      • StainWorks software: a centralized app for accessing and configuring protocols
      • Reagent Layout files
      • Running the "Blue Dye Demo" protocol
      • Copy of CODEX (PhenoCyler™)
        • CODEX Reagent Setup
      • Temperature-controlled incubations
      • Page 2
      • ParheliaBio.com content
        • Advanced Tissue Staining Solutions | ParheliaBio
        • Omni-Stainer™ S12 - Automation Bundle
        • Omni-Stainer™ S12 - Manual Operation Bundle
        • Shop
        • Omni-Stainer™ S12 Thermal Sheath
        • Omni-Stainer™ C12 - Automation Bundle
        • Omni-Stainer™ C12 Thermal Sheath
        • Consumables Archives
        • Conditioning Reagent 5ml Bottle
        • Omni-Stainer™ C12 - Manual Operation Bundle
        • S12 Coverpads - 48 Pack
        • C12 Filter Paper
        • Opentrons Robots Archives
        • S12 Filter Paper
        • C12 Support Pad - 48 Pack
        • Opentrons OT-2 Liquid Handling Robot
        • Opentrons Single-Channel Electronic Pipette p20 (GEN2)
        • Opentrons 300 µl Pipette Tip Tip Racks - 9600 tips
        • Greater Tissue Staining Workflow Efficiency. Slide Staining Kits
      • Opentrons setup guides
        • Opentronssetupguides
        • GetstartedImportaprotocol
      • More Parheliabio.com content
        • Howitworks
        • Parhelia Biosciences FAQs
          • Page 1
            • Frequently asked questions parheliabio website
        • ParheliaFrequentlyaskedquestions
        • Parhelia Omni-Stainer FAQ
      • Meet your Parhelia Omnistainer
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  • Opentrons OT-2 Robot and Software setup
  • Checklist

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  1. Product Guides

Automation (Opentrons OT-2) Setup

This page goes through setting up a new Opentrons OT-2, most of the content is in links to Opentrons website and Opentrons videos.

PreviousManual Operation and "Blue Dye Demo" Manual ProtocolNextOmni-Stainer Automation Overview

Last updated 1 year ago

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This is for automation bundles only, click here for manual operation:Manual Operation and "Blue Dye Demo" Manual Protocol

If you want to get a head start, download our GitHub archive from !

Opentrons OT-2 Robot and Software setup

^ This playlist covers ^

Checklist

The Opentrons Help Center at is a fantastic resource that has s as well as (which we strongly recommend) like this video series below that takes you from box to protocol.

The Deck Calibration process in video 5 of jogging the pipette tip in the XYZ directions into the center of the engraved "+" symbol on the Opentrons deck is very similar to the labware calibration for the Omni-Stainer™ which is performed using the embossed "+" symbol on the C12 or S12 Omni-Stainer™ lid.

Our engaging virtual demonstration serves as an excellent introductory guide for understanding the steps in operating the Parhelia Bio Omni-Stainer C12 and S12, encompassing both manual and automated usage, along with thermal sheath and thermal modules.

Set up Opentrons OT-2 robot. , and our friends at Opentrons are happy to assist you with this if you have any .

If you would prefer to visit the GitHub and download individual files our GitHub is at

Download customized or stock Omni-stainer script from

https://support.opentrons.com/s/
tep-by-step guides
video guides
Unboxing the OT-2
Unlocking the OT-2
Setting up the Opentrons App
Attaching Pipettes to the OT-2
Deck Calibration on the OT-2
Tip Length & Pipette Offset Calibration on the OT-2
Running a Protocol on the OT-2
As in this video
View the virtual demo here!
This set-up guide is a great reference
questions
Download Opentrons App
Download Parhelia Bio's base OT-2 scripts and labware definitions from GitHub by clicking here
https://github.com/parheliabio/ot2-scripts
Stainworks
🤖
here
Opentrons Help Center
^ Click here ^ For Opentrons Set up
Logo
Example of staining protocol loaded into Opentrons
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